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Rapid Characterization of Genetic Parts with Cell-Free Systems
Published on: August 30, 2021
Genetic characterization of a hantavirus isolated from Heilongjiang province, China
Yulong Wang1, Suya Cao1, Cheng Cheng1
1Northeast Forestry University, 26 Hexing Road, Harbin, 150040, China (Wang, Cao, Hua); Heilongjiang International Travel Healthcare Center, Harbin, 150000, China (Cheng, Ju).
Abstract:
Hantavirus is the causative agent of hemorrhagic fever with renal syndrome (HFRS). Heilongjiang Province is experiencing an epidemic of HFRS, the main causative agent is a variant of hantavirus called Seoul virus (SEOV). In this study, the entire genome of one SEOV, the DN2 strain, was sequenced and analyzed. The alignment analysis of the sequences indicated that the DN2 strain shares the highest homology with the SEOV-LYO852 strain. The nucleotide identity is 97.6% for the S segment, 97.7% for the M segment, and 98.0% for the L segment. The corresponding amino acid sequence homologies are 99.1%, 98.9% and 99.8%. The phylogenetic analysis of the segments suggests that the DN2 strain has a high genetic relationship with SEOV strains and no genetic recombination occurs.
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