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A protocol for generating induced T cells by reprogramming B cells in vivo.
Qitong Weng1,2,3,4,5, Fangxiao Hu1,2,3,5, Mengyun Zhang1,2,3,4,5
1CAS key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.
Cell Regeneration (London, England)
|January 24, 2019
Summary
Researchers developed a reproducible method to generate functional T cells from Hoxb5-expressing pro/pre-B cells in vivo. This breakthrough offers a new approach for induced T cell generation in regenerative medicine and translational research.
Area of Science:
- Immunology
- Regenerative Medicine
- Cell Biology
Background:
- Reprogramming cells to obtain T cells is a key objective in regenerative medicine.
- Existing methods for T cell generation face challenges in efficiency and applicability.
Purpose of the Study:
- To establish a protocol for generating functional T cells from Hoxb5-expressing pro/pre-B cells in vivo.
- To provide a reproducible and straightforward method for induced T cell generation.
Main Methods:
- Construction of Hoxb5 recombinant plasmids.
- Retroviral packaging and isolation of pro/pre-B cells.
- Viral transduction, cell transplantation, and phenotypic analysis of induced T cells.
Main Results:
- Successfully generated functional T cells from Hoxb5-expressing pro/pre-B cells in vivo.
- The protocol is demonstrated to be reproducible and straightforward.
- The generated T cells are suitable for translational research.
Conclusions:
- A novel protocol for in vivo T cell generation from pro/pre-B cells has been established.
- This method provides a viable approach for producing induced T cells for therapeutic applications.
- The findings contribute to advancements in regenerative medicine and T cell-based therapies.
Keywords:
7-AAD, 7-Aminoactinomycin DAPC, AllophycocyaninBV, Brilliant VioletCAR-T, Chimeric antigen receptor T-Cell ImmunotherapyDAPI, 4,6-diamidino-2-phenylindoleDN, double negativeFITC, Fluorescein isothiocyanateGFP, green fluorescent proteinHSC, Hematopoietic stem cellsHoxb5LN, Lymph nodePB, peripheral bloodPE, PhycoerythrinPerCP, Peridinin ChlorophyllPro/pre-B cellsRetrovirusSP, single positiveT cellsTAA-TCR-T, tumor-associated antigen-TCR-TiPSC, induced pluripotent stem cellsMore Related Videos
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