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Updated: Jan 30, 2026

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
Published on: June 27, 2022
An endoplasmic reticulum-targetable fluorescent probe for highly selective detection of hydrogen sulfide
Hui Zhang1, Jiali Chen, Haiqing Xiong
1College of Chemistry & Chemical Engineering, Central South University, Changsha, Hunan 410083, P. R. China. xzsong@csu.edu.cn.
Abstract:
Hydrogen sulfide (H2S), a critical endogenous signaling molecule, is widely involved in many physiological processes. Endoplasmic reticulum, an important organelle with a sac-like structure, plays crucial roles in maintaining the normal function of cells. Accordingly, monitoring the H2S levels in endoplasmic reticulum is of great importance. Herein, we have developed an endoplasmic reticulum-targetable fluorescent probe, ER-CN, for H2S detection. ER-CN features excellent sensing properties, such as high sensitivity and selectivity. In addition, ER-CN exhibits low cytotoxicity and a fine endoplasmic reticulum targeting property (with a Pearson's colocalization coefficient of 0.95). Significantly, visualization of H2S in the endoplasmic reticulum of living HeLa cells by using ER-CN was successfully realized.
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