Related Experiment Video
Updated: Jan 30, 2026

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart
Published on: September 15, 2018
In Vivo Light Sheet Fluorescence Microscopy of Calcium Oscillations in Arabidopsis thaliana
Neli Romano Armada1,2, Fabrizio Gandolfo Doccula3, Alessia Candeo1
1Dipartimento di Fisica, Politecnico di Milano, Milan, Italy.
Abstract:
Calcium imaging in plants requires a high-resolution microscope, able to perform volumetric acquisition in a few seconds, inducing as low photobleaching and phototoxicity as possible to the sample. Light sheet fluorescence microscopy offers these capabilities, with the further chance to mount the sample in vertical position, mimicking the plant's growth and physiological conditions.A protocol for plant preparation and mounting in a light sheet microscope is presented. First, the growth of Arabidopsis thaliana in a sample holder compatible with light sheet microscopy is described. Then, the requirements for sample alignment and image acquisition are detailed. Finally, the image processing steps to analyze calcium oscillations are discussed, with particular emphasis on ratiometric calcium imaging in Arabidopsis root hairs.
Related Concept Videos
Confocal Fluorescence Microscopy
Light as Energy
Photons
A photon is a discrete electromagnetic particle or bundle of energy. Photons are characterized by their frequency, wavelength, and amplitude, similar to the properties of a wave. Waves with higher frequencies transmit more energy and have shorter wavelengths than longer wavelengths that transmit...
Oscillations In An LC Circuit
Forced Oscillations
Damped Oscillations
Although friction and other non-conservative...
Super-resolution Fluorescence Microscopy

