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Isolation and Identification of Myricitrin, an Antioxidant Flavonoid, from Daebong Persimmon Peel

In-Wook Hwang1, Shin-Kyo Chung2

  • 1Department of Food Science and Nutrition, Dong-A University, Busan 49315, Korea.

Preventive Nutrition and Food Science
|January 25, 2019
PubMed

Insights

Daebong persimmon peels contain the antioxidant flavonoid myricitrin. This compound demonstrated significant antioxidant activity, suggesting its role in the peel

Area of Science:

  • Phytochemistry
  • Natural Products Chemistry
  • Food Science

Background:

  • Persimmon (Diospyros) fruits are known for their rich nutritional and phytochemical profiles.
  • Persimmon peels, often discarded, are a potential source of bioactive compounds with health benefits.
  • Antioxidants play a crucial role in combating oxidative stress and related diseases.

Purpose of the Study:

  • To isolate and identify antioxidant compounds from Daebong persimmon peels.
  • To evaluate the antioxidant potential of the isolated compounds.
  • To determine the major antioxidant flavonoid responsible for the activity in persimmon peel extract.

Main Methods:

  • Sequential solvent extraction (n-hexane, ethyl acetate, n-butanol) of persimmon peel.
  • Fractionation using silica gel and octadecylsilane column chromatography.
  • Purification and identification using High-Performance Liquid Chromatography (HPLC), Liquid Chromatography/Mass Spectrometry (LC/MS), and Nuclear Magnetic Resonance (NMR) spectroscopy (1H and 13C).
  • Antioxidant activity assays including Ferric Ion Reducing Antioxidant Power (FRAP) and alpha,alpha-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging.

Main Results:

  • The ethyl acetate fraction exhibited the highest antioxidant activity.
  • Three antioxidant compounds were isolated from the ethyl acetate fraction.
  • Compound 2 was identified as myricitrin using spectroscopic and spectrometric techniques.
  • Myricitrin displayed potent antioxidant activity in both FRAP and DPPH assays.

Conclusions:

  • Myricitrin is a major antioxidant flavonoid present in Daebong persimmon peels.
  • The strong antioxidant capacity of Daebong persimmon peel is attributed significantly to myricitrin.
  • These findings highlight the potential of persimmon peels as a source of natural antioxidants for food and nutraceutical applications.
Keywords:
LC/MSNMRantioxidantmyricitrinpersimmon peel

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