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Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI
Published on: March 24, 2016
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Deep-ultraviolet laser ablation electrospray ionization mass spectrometry.
Remilekun O Lawal1, Fabrizio Donnarumma1, Kermit K Murray1
1Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana, USA.
Journal of Mass Spectrometry : JMS
|January 25, 2019
Summary
Deep UV laser ablation electrospray ionization mass spectrometry enables analysis of biological samples. This method detects peptides, proteins, and lipids without fragmentation, offering a novel approach for molecular analysis.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Mass Spectrometry
Background:
- Conventional electrospray ionization mass spectrometry (ESI-MS) is a powerful tool for analyzing biomolecules.
- Limitations exist in sample preparation and ionization efficiency for certain biological samples.
Purpose of the Study:
- To develop and evaluate a novel ambient laser ablation electrospray ionization mass spectrometry (LAESI-MS) method using deep ultraviolet (DUV) laser.
- To assess the applicability of DUV-LAESI-MS for the analysis of peptides, proteins, and lipids in biological samples.
Main Methods:
- Utilized a 193-nm wavelength pulsed ArF excimer laser for ambient laser ablation of solid biological samples.
- Integrated the ablated plume with charged electrospray droplets for ion formation.
- Analyzed ions using a quadrupole time-of-flight mass spectrometer.
Main Results:
- Successfully detected peptides and proteins up to 66-kDa molecular weight without observable fragmentation.
- Identified multiply charged ions in mass spectra, consistent with conventional ESI.
- Enabled direct detection of lipids from untreated biological tissue samples.
Conclusions:
- DUV-LAESI-MS is a viable technique for sensitive and direct analysis of biomolecules in biological samples.
- The method minimizes fragmentation, preserving molecular integrity.
- Potential ionization mechanisms involve laser energy absorption by analyte molecules or residual water.
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