Related Experiment Video
Updated: Jan 30, 2026
09:05
Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
Published on: March 11, 2020
12.6K
Tomato spotted wilt virus Can Infect Resistant Tomato when Western Flower Thrips Inoculate Blossoms
1Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, 27695-7630.
Plant Disease
|January 25, 2019
Summary
Tomato spotted wilt virus (TSWV) can infect resistant tomato plants through blossoms, even with the Sw-5 allele. Managing thrips during flowering is crucial for controlling TSWV in all tomato cultivars.
Area of Science:
- Plant Pathology
- Entomology
- Genetics
Background:
- Tomato spotted wilt is a significant global crop disease.
- The Sw-5 allele in tomato (Solanum lycopersicum) confers resistance to Tomato spotted wilt virus (TSWV).
- Infections in Sw-5+ plants suggest incomplete resistance expression in reproductive tissues.
Purpose of the Study:
- To investigate if western flower thrips (Frankliniella occidentalis) can transmit TSWV to blossoms of Sw-5+ and Sw-5- tomato plants.
- To determine the role of floral inoculation in TSWV transmission and systemic infection.
Main Methods:
- Adult thrips were used to inoculate blossoms and small fruit of Sw-5+ and Sw-5- tomato plants.
- Infection rates in reproductive tissues and systemic infections were assessed.
- Field experiments were conducted to validate findings under natural conditions.
Main Results:
- Twenty-one percent of Sw-5+ plants and 68% of Sw-5- plants developed infections after thrips inoculation of blossoms/fruit.
- Systemic infections occurred in both Sw-5+ and Sw-5- genotypes following blossom inoculation.
- Field studies confirmed high rates of fruit infection and limited foliage infection in Sw-5+ plants during thrips abundance.
Conclusions:
- TSWV transmission through blossoms by thrips can lead to infections in Sw-5+ tomato plants.
- Floral inoculation is a viable pathway for TSWV infection, impacting both resistant and susceptible cultivars.
- Effective management of Frankliniella occidentalis during tomato flowering is essential for TSWV control.
Related Concept Videos
02:40Pollination and Flower Structure
75.5K
Flowers are the reproductive, seed-producing structures of angiosperms. Typically, flowers consist of sepals, petals, stamens, and carpels. Sepals and petals are the vegetative flower organs. Stamens and carpels are the reproductive organs.
75.5K
01:15Western Blotting
20.6K
Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
20.6K
00:50What are Viruses?
128.1K
Overview
128.1K
01:22Resistivity
4.5K
When a voltage is applied to a conductor, an electrical field is generated, and charges in the conductor feel the force due to the electrical field. The current density that results depends on the electrical field and the properties of the material. In some materials, including metals at a given temperature, the current density is approximately proportional to the electrical field. In these cases, the current density can be modeled as:
4.5K
01:19Resistance
6.0K
When a current moves through any conductor, the conductor causes some level of difficulty for the current to flow. The measure of that difficulty is known as the resistance of the material and is represented by R. Every material has its own resistance. In the case of conductors, heat is emitted whenever a current passes through them. Resistance depends on the resistivity of the material. Resistivity is a characteristic of the material used to fabricate electrical components, whereas the...
6.0K
01:16Equivalent Resistance
977
In circuit analysis, situations often arise where resistors are neither in series nor parallel configurations. To tackle such scenarios, three-terminal equivalent networks like the wye (Y) (Figure 1 (a)) or tee (T) and delta (Δ) (Figure 1 (b)) or pi (π) networks come into play. These networks offer versatile solutions and are frequently encountered in various applications, including three-phase electrical systems, electrical filters, and matching networks.
977