Related Experiment Video
Updated: Jan 30, 2026

A Simple and Efficient Method to Detect Nuclear Factor Activation in Human Neutrophils by Flow Cytometry
Published on: April 9, 2013
[Relationship between nuclear factor-κB signaling pathway and susceptibility of recurrent aphthous ulcer]
Jing Zhang1, Rui-Qi Linghu, Jing-Jing Sha
1Department of Endodontics, Stomatological Hospital of Ningxia Medical University General Hospital. Yinchuan 750004, Ningxia Hui Autonomous Region, China.
Purpose:
To explore the possible relationship between recurrent aphthous ulcer (RAU) and nuclear factor-κB signaling pathway.
Methods:
A total of 124 RAU patients were recruited for this study. The control group consisted of 133 healthy subjects. Serum NFκBp50, NFκBp65, IκBα and IKK concentration were detected by ELISA.NFκB-94 ins/del ATTG sites were detected by restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR). Relative risk ratios were estimated by odds ratios (OR) and 95% confidence interval (95%CI). Statistical analysis was performed using SPSS 20.0 software package.
Results:
Serum NFκBp50, NFκBp65 and IKK levels in RAU patients were significantly lower than those of the controls (P<0.05). Serum IκBα level in RAU patients was significantly higher than those of the controls (P<0.05). Significant differences were found in the genotype frequencies or allele frequencies of NFκB-94 ins/del ATTG sites between RAU patients and controls (P<0.05). ID genotype(OR=3.073,95%CI=1.557-6.067), DD genotype (OR=4.851,95%CI=2.264-10.393), and D allele (OR=2.079,95%CI=1.462-2.957) at NFκB-94 ins/del ATTG site exhibited high risks of RAU.
Conclusions:
NF kappa B signaling pathway is associated with RAU.NFκB-94 ins/del ATTG sites are associated with higher risk of RAU. NFκB-94 ins/del ATTG D allele may serve as genetic determinants for RAU.
Related Concept Videos
NF-κB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The...
Notch Signaling Pathway
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
Hedgehog Signaling Pathway
Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Pathophysiology of Peptic Ulcer Disease: Injurious Factors
In the antrum region, G cells secrete the gastrin hormone that binds to gastrin-cholecystokinin-B (CCK2) receptors on parietal and enterochromaffin-like (ECL) cells in the fundic glands. Simultaneously, the vagus nerve releases acetylcholine, which binds...
Non-Canonical Wnt Signaling Pathways

