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Related Experiment Video

Updated: Jan 30, 2026

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

Illumination conditions in microsphere-assisted microscopy.

Stephane Perrin¹, Hongyu Li¹, Audrey Leong-Hoi¹

¹ICube, University of Strasbourg - UMR CNRS 7357, Photonics Instrumentation and Processes, Illkirch, France.

Journal of Microscopy

|January 26, 2019

Summary

This summary is machine-generated.

White-light microsphere-assisted microscopy achieves subdiffraction resolution. Optimizing illumination conditions, including diaphragm apertures and light source spectral width, is crucial for enhancing contrast and depth-of-focus.

Keywords:

Illumination Imaging depth Microsphere Optical transfer function Super-resolution microscopy

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Area of Science:

Optical microscopy
Super-resolution imaging
Label-free imaging

Background:

White-light microsphere-assisted microscopy offers label-free, full-field imaging with subdiffraction lateral resolution.
The technique's performance is sensitive to both geometrical parameters and illumination conditions.

Purpose of the Study:

To investigate the impact of Köhler illumination parameters on microsphere-assisted microscopy.
To determine how diaphragm apertures and light source spectral width affect depth-of-focus and imaging contrast.

Main Methods:

Experimental measurements and computer simulations were conducted in air.
The optical transfer function was measured to analyze optical aberrations.

Main Results:

The influence of diaphragm apertures and spectral width on depth-of-focus and contrast was quantified.
The super-resolution phenomenon was successfully demonstrated.
Optical aberrations were characterized for various illumination arrangements.

Conclusions:

Illumination conditions significantly influence the performance of white-light microsphere-assisted microscopy.
Optimized illumination parameters are essential for maximizing contrast and depth-of-focus.
This study provides insights into controlling and enhancing super-resolution imaging with microspheres.