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High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
Published on: November 16, 2019
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A novel dual-color total internal reflection fluorescence detecting platform using compact optical structure and
Dan Song1, Rong Yang1, Shunyan Fang1
1School of Environment and Natural Resources, Renmin University of China, Beijing 100872, China.
Talanta
|January 27, 2019
Summary
A new dual-color fluorescence detecting platform (DTP) enables simultaneous detection of two dyes with high sensitivity. This cost-effective system offers a potential alternative for advanced fluorescence detection applications.
Area of Science:
- Optoelectronics
- Biomedical Instrumentation
- Analytical Chemistry
Background:
- Conventional dual-color fluorescence detection systems often face limitations in sensitivity and complexity.
- Simultaneous detection of multiple fluorescent signals is crucial for various biological and chemical analyses.
- Miniaturization and integration of optical detection platforms are key trends in modern analytical instrumentation.
Purpose of the Study:
- To develop a novel dual-color total internal reflection fluorescence detecting platform (DTP) for simultaneous detection of two fluorescence dyes.
- To enhance optical efficiency and detection sensitivity using a compact optical system and a Si-based photodetector.
- To demonstrate the platform's capability for sensitive and cost-effective dual-color fluorescence detection.
Main Methods:
- Development of a DTP utilizing a simple optical system with a single-multimode fiber optic coupler.
- Implementation of a fiber optical switch to alternatively excite two wavelength fluorescence dyes.
- Employment of a Si-based photodetector (SOP-1000) for time-resolved signal detection.
- Characterization of the DTP's sensitivity and limit of detection for specific dyes.
Main Results:
- The DTP achieved simultaneous detection of two fluorescence signals with high sensitivity (50 fW light intensity).
- Demonstrated limits of detection for Cy5.5 and Pacific Blue dye (PB) were 0.05 nM and 2.1 nM, respectively.
- The system exhibited negligible cross-talk and a cost-effective design.
Conclusions:
- The developed miniaturized and integrated DTP offers high sensitivity, a simple optical structure, and cost-effectiveness.
- This DTP presents a viable alternative to conventional dual-color fluorescence detecting systems.
- The platform holds potential as a component in micro-total analysis systems (μ-TAS) for sensitive dual-color fluorescence detection.
Keywords:
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