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Updated: Jan 30, 2026

Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles
Published on: October 4, 2012
Application of functionalized silver nanoparticles as a biochemical sensor for selective detection of lysozyme
Kamlesh Shrivas1, Nidhi Nirmalkar2, Manas Kanti Deb3
1School of Studies in Chemistry, Pt. Ravishankar Shukla University, Raipur CG-492010, India; Department of Chemistry, Guru Ghasidas Vishwavidyalaya, Koni, Bilaspur CG-495009, India.
Abstract:
Silver nanoparticles (AgNPs) functionalized with glutamic acid (GA) was used as a biochemical sensing probe in colorimetry for detection of lysozyme protein in milk samples. The method is based on the color change of AgNPs/GA from yellow to reddish-yellow differentiated with naked eyes for qualitative determination and red shift of localized surface plasmon resonance (LSPR) absorption signal intensity of AgNPs/GA in visible region used for quantitative determination of lysozyme. The control experiments were performed to demonstrate the electrostatic force of interactions between AgNPs/GA and protein molecule. A wide linear range of 3-150 nM with limit of detection of 1.5 nM was acquired for quantitative determination of lysozyme using AgNPs/GA as a biochemical sensing probe. The advantages of using AgNPs/GA as a biochemical sensing probe are simple, label-free and economic for determination of lysozyme from milk samples.
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