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Updated: Jan 30, 2026

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
Published on: October 24, 2019
Evaluation of different soft tissue-simulating materials in pixel intensity values in cone beam computed tomography
Gustavo Machado Santaella1, Maria Augusta Portella Guedes Visconti2, Karina Lopes Devito3
1Department of Oral Diagnosis, Division of Oral Radiology, Piracicaba Dental School, State University of Campinas, Piracicaba, São Paulo, Brazil.
Expanded polystyrene (EPS) and utility wax effectively simulated soft tissues for cone beam computed tomography (CBCT) imaging in most regions. These materials offer a viable alternative for simulating soft tissues in dental imaging research.
Area of Science:
- Dental Imaging
- Biomaterials Science
Background:
- Accurate simulation of soft tissues is crucial for interpreting cone beam computed tomography (CBCT) images in dental research.
- Understanding the influence of soft tissues on CBCT image quality is essential for diagnostic accuracy.
Purpose of the Study:
- To evaluate the efficacy of various materials as soft tissue simulators in CBCT.
- To assess the impact of intact soft tissues versus simulated tissues on CBCT imaging outcomes.
Main Methods:
- Piglet heads were imaged with intact soft tissues, stripped tissues, and four different simulated soft tissue materials (acrylic, water, utility wax, expanded polystyrene).
- Pixel intensity values were analyzed for teeth and alveolar bone regions using analysis of variance (ANOVA).
Main Results:
- Expanded polystyrene (EPS) with or without utility wax demonstrated effectiveness in simulating soft tissues for most evaluated regions.
- Acrylic showed moderate effectiveness, while water proved ineffective as a soft tissue simulator.
- Statistical differences were noted in the lower anterior bone and upper anterior tooth regions across various material groups.
Conclusions:
- EPS (2-cm thick), with or without utility wax (1-cm thick), is a suitable material for simulating soft tissues in CBCT imaging for most anatomical areas.
- Acrylic (0.5 cm) is a less effective but viable alternative.
- The influence of intact soft tissues on CBCT image quality was minimal in most regions evaluated.
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