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Related Experiment Video

Updated: Jan 30, 2026

Analysis of Histone Antibody Specificity with Peptide Microarrays

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Published on: August 1, 2017

Virus assay using antibody-functionalized peptide nanotubes.

Robert I MacCuspie¹, Ipsita A Banerjee¹, Christophe Pejoux¹

¹Department of Chemistry, City University of New York, Huner College and the Graduate Center, 695 Park Ave, New York, NY, 10065, USA; ; Tel: +1 (212) 650 3918.

|January 29, 2019

Summary

A new fluorescent antibody nanotube method enables rapid, highly sensitive detection of viruses at trace levels. This breakthrough offers crucial advancements for disease control and bioterrorism threat detection.

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Peptide-based Identification of Functional Motifs and their Binding Partners

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

Area of Science:

Nanotechnology
Virology
Biochemistry

Background:

Robust trace-level virus detection is critical for public health and security.
Existing methods may lack the required speed, sensitivity, or specificity.
Urgent needs exist for rapid and sensitive pathogen detection.

Purpose of the Study:

To develop a novel method for rapid and highly sensitive virus detection.
To utilize fluorescent antibody nanotubes for pathogen identification.
To demonstrate the assay's effectiveness for trace-level virus quantification.

Main Methods:

Utilized fluorescent antibody nanotubes that aggregate around target viruses.
Measured changes in fluorescence and light scattering intensities upon nanotube aggregation.
Tested the assay with various viruses including herpes simplex virus type 2, adenovirus, vaccinia, and influenza type B.
Validated specificity by quantifying mixtures of two different viruses.

Main Results:

Achieved attomolar-level detection of multiple viruses.
Demonstrated rapid aggregation of antibody nanotubes around viral pathogens.
Confirmed high specificity of antibody nanotubes for targeted viruses.
Successfully detected viruses within 30 minutes of incubation.

Conclusions:

The fluorescent antibody nanotube assay provides a rapid and highly sensitive method for virus detection.
This technique can effectively detect and quantify viruses at trace levels.
The assay holds significant potential for applications in disease surveillance and bioterrorism response.