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Microfluidic Applications for Disposable Diagnostics
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Impedimetric array in polymer microfluidic cartridge for low cost point-of-care diagnostics.

Andrew Lakey1, Zulfiqur Ali1, Simon M Scott1

  • 1Healthcare Innovation Centre, Teesside University, Middlesbrough, Tees Valley TS1 3BX, UK.

Biosensors & Bioelectronics
|January 29, 2019
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Summary

This study presents a low-cost microfluidic device for detecting D-dimer, a biomarker for deep vein thrombosis and pulmonary embolism (DVT/PE). This point-of-care diagnostic tool offers a rapid and accessible method for identifying DVT/PE in patients.

Keywords:
ElectropolymerisationImpedimetric sensingInterdigitated electrodesMicrofluidicsPoint-of-care diagnostics

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Clinical Diagnostics

Background:

  • Deep Vein Thrombosis and pulmonary embolism (DVT/PE) are significant causes of in-hospital mortality.
  • D-dimer is a crucial blood biomarker for DVT/PE diagnosis.
  • Current diagnostic methods may lack point-of-care accessibility and cost-effectiveness.

Purpose of the Study:

  • To develop a low-cost, disposable microfluidic device for point-of-care (POC) impedimetric detection of D-dimer.
  • To biofunctionalize an interdigitated electrode (IDE) array for sensitive D-dimer detection.
  • To demonstrate the feasibility of this approach for rapid DVT/PE diagnosis.

Main Methods:

  • Fabrication of IDE arrays on a polyethylenenaphtalate (PEN) substrate.
  • In situ biofunctionalization of IDEs via electropolymerization of polypyrrole copolymer.
  • Immobilization of histidine tag anti-D-Dimer antibody onto the copolymer.
  • Optimized copolymer film production using chronopotentiometry (5μA, 50s).
  • Quality control via optical microscopy, chronopotentiometry, and impedimetric analysis.

Main Results:

  • Consistent copolymer films achieved with optimized chronopotentiometry.
  • Successful biofunctionalization monitored through various techniques.
  • Detection of D-dimer in clinical plasma samples (437 ng/mL) yielded a ratiometric reading of 124 ± 15% (95% CI).
  • Demonstrated proof-of-concept for impedimetric detection using functionalized IDEs.

Conclusions:

  • A low-cost, disposable microfluidic device for POC D-dimer detection has been successfully developed.
  • The biofunctionalized IDE array enables sensitive impedimetric analysis.
  • This platform technology is adaptable for detecting various analytes, offering a versatile POC diagnostic solution.