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Live sperm trap microarray for high throughput imaging and analysis.

Jae Bem You1, Yihe Wang, Christopher McCallum

  • 1Department of Mechanical and Industrial Engineering, University of Toronto, 5 King's College Road, Toronto, ON M5S 3G8, Canada. sinton@mie.utoronto.ca.

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Researchers developed a 103-trap microarray to analyze individual live sperm. This technology enables high-throughput single-cell analysis of sperm motility and DNA integrity, crucial for understanding male fertility.

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Area of Science:

  • Reproductive Biology
  • Cell Biology
  • Biotechnology

Background:

  • Male fertility research traditionally relies on population-level data, overlooking single-cell variability crucial for fertilization.
  • Existing single-cell analysis methods are limited for highly motile sperm, hindering detailed investigation.
  • The 'winner-takes-all' nature of fertilization necessitates understanding individual sperm characteristics.

Purpose of the Study:

  • To develop a novel method for trapping, aligning, and analyzing individual live sperm at high resolution.
  • To enable simultaneous assessment of sperm motility, DNA integrity, and mitochondrial activity at the single-cell level.
  • To investigate potential correlations between sperm motility and DNA integrity at the single-cell level.

Main Methods:

  • A 103-trap microarray system was designed to immobilize and array individual live sperm.
  • High-resolution imaging was used to analyze sperm head morphology and quantify tail beat characteristics (frequency, amplitude).
  • Dye-based staining (acridine orange) assessed DNA integrity, and mitochondrial activity was also evaluated.

Main Results:

  • The microarray successfully trapped and aligned individual live sperm for comprehensive analysis.
  • No statistically significant correlation was found between sperm tail beat characteristics and DNA integrity at the single-cell level.
  • The system facilitated high-throughput, single-cell resolution analysis of multiple sperm parameters.

Conclusions:

  • The developed trap array is a powerful tool for high-throughput, single-cell analysis of live sperm.
  • This technology offers unprecedented insights into the individual characteristics of sperm relevant to fertilization.
  • Findings challenge sample-level observations by revealing no direct correlation between motility and DNA integrity in individual sperm.