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Updated: Jan 30, 2026

Use of Galleria mellonella as a Model Organism to Study Legionella pneumophila Infection
Published on: November 22, 2013
Quantification of Viable but Non-Culturable Cells of Legionella pneumophila
Rosalie S Braun1,2, Nilmini Mendis2, Laam Li2,3
1New York University College of Dentistry, New York, NY, USA.
Abstract:
Legionella pneumophila, among other bacteria, may enter a viable but non-culturable state as a means for survival in stressful conditions. Bacterial cells in the viable but non-culturable state cannot grow on standard medium; however, they continue to exhibit characteristics that are associated with live cells, such as respiration, transcription, and cell wall integrity. The present paper outlines a detailed protocol for the detection of viable but non-culturable L. pneumophila cells via Syto® 9 and propidium iodide staining coupled with flow cytometry.
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