Four new compounds from Neoboletus magnificus

Area of Science:

• Natural Product Chemistry
• Pharmacognosy
• Medicinal Mycology

Background:

• The mushroom *Neoboletus magnificus* is a source of bioactive natural products.
• Natural products are increasingly investigated for therapeutic properties, including anti-inflammatory effects.

Purpose of the Study:

• To isolate and characterize chemical constituents from the fruiting body of *Neoboletus magnificus*.
• To evaluate the *in vitro* anti-inflammatory activity of the isolated compounds.

Main Methods:

• Methanol extraction of the *Neoboletus magnificus* fruiting body.
• Structure elucidation using High-Resolution Mass Spectrometry (HRMS) and Nuclear Magnetic Resonance (NMR) spectroscopy.
• In vitro assays to assess anti-inflammatory activity.

Main Results:

• Four new compounds (1, 2, 4, 6) and two known compounds (3, 5) were successfully isolated.
• The chemical structures of all isolated compounds were determined.
• The anti-inflammatory potential of the isolated compounds was evaluated *in vitro*.

Conclusions:

• The study successfully identified novel and known compounds from *Neoboletus magnificus*.
• The isolated compounds represent potential leads for the development of new anti-inflammatory agents.
• Further research is warranted to explore the therapeutic applications of these compounds.