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Tractable Mammalian Cell Infections with Protozoan-primed Bacteria

Tractable Mammalian Cell Infections with Protozoan-primed Bacteria

Published on: April 2, 2013

Selection of self-priming molecular replicators.

Daechan Park¹, Andrew D Ellington², Cheulhee Jung³

¹Department of Biological Sciences, College of Natural Sciences, Ajou University, Suwon 16499, Republic of Korea.

Nucleic Acids Research

|January 31, 2019

Summary

This summary is machine-generated.

Early life replicators likely emerged through self-priming amplification. This study shows how specific sequences, particularly those favoring purine insertion, dominated in early replication competition, outcompeting less efficient variants.

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Area of Science:

Origin of life studies
Molecular evolution
Biochemistry

Background:

Oligonucleotide amplification can occur via self-priming and concatemerization.
Phosphorothioate linkages facilitate these processes.
Understanding early replicator competition is key to origin of life research.

Purpose of the Study:

To investigate the competitive dynamics of early replicators.
To identify sequence features that confer a selective advantage during replication.
To model potential mechanisms for the emergence of life.

Main Methods:

Selection experiments using a library of phosphorothiolated hairpins with random sequences (N10).
Inclusion of deoxynucleoside triphosphates and polymerase for amplification.
Serial transfer of amplified products to enrich for dominant sequences.

Main Results:

Rapid formation of concatemers was observed.
Sequences templating purine insertion, especially during initiation, predominated.
Specific sequences accumulated over serial transfers, demonstrating competitive advantage.

Conclusions:

Self-priming amplification is a viable mechanism for early replicator accumulation.
Sequence-dependent templating, particularly purine preference, drove early replicator competition.
This mechanism provides insight into how specific replicators may have emerged and persisted near the origin of life.