Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Noncompartmental Analysis: Miscellaneous Pharmacokinetic Parameters

Noncompartmental Analysis: Miscellaneous Pharmacokinetic Parameters

The noncompartmental approach is a widely used method in pharmacokinetics to assess drugs' behaviors in the body. It considers several factors, including clearance, bioavailability, and total volume of distribution.
One key aspect of the noncompartmental approach is determining a drug's total clearance. This can be done by dividing the drug dose by the area under the concentration-time curve from zero to infinity. The area under the concentration-time curve represents the drug's...

Wave Parameters

Wave Parameters

The simplest mechanical waves are associated with simple harmonic motion and repeat themselves for several cycles. These simple harmonic waves can be modeled using a combination of sine and cosine functions. Consider a simplified surface water wave that moves across the water's surface. Unlike complex ocean waves, in surface water waves, water moves vertically, oscillating up and down, whereas the disturbance of the wave moves horizontally through the medium. If a seagull is floating on the...

Distributions to Estimate Population Parameter

Distributions to Estimate Population Parameter

The accurate values of population parameters such as population proportion, population mean, and population standard deviation (or variance) are usually unknown. These are fixed values that can only be estimated from the data collected from the samples. The estimates of each of these parameters are sample proportion, the sample mean, and sample standard deviation (or variance). To obtain the values of these sample statistics, data are required that have particular distribution and central...

Dosage Regimens: Partial Pharmacokinetic Parameters

Dosage Regimens: Partial Pharmacokinetic Parameters

It is not uncommon for complete drug pharmacokinetic profiles to remain elusive in pharmacokinetics. This necessitates certain educated assumptions by pharmacokineticists to determine appropriate dosage regimens without comprehensive pharmacokinetic data from animal or human studies. One prevalent assumption is setting the bioavailability factor, denoted as F, to 1 or 100%. This assumption caters to the scenario where a drug doesn't achieve full systemic absorption, resulting in the patient...

Factors Influencing Drug Absorption: Anatomical Parameters

Factors Influencing Drug Absorption: Anatomical Parameters

Drug absorption involves the movement of drugs from the point of administration into the systemic circulation. Initially, Gastrointestinal (GI) motility propels the drug through the digestive tract and into the stomach. However, the stomach's high acidity and limited surface area restrict its role in drug absorption for most drugs. The drug then moves from the stomach to the small intestine via gastric emptying, which can be slowed by various factors, including interactions with other...

Factors Influencing Drug Absorption: Physicochemical Parameters

Factors Influencing Drug Absorption: Physicochemical Parameters

The physicochemical characteristics of drugs play a crucial role in formulating stable and bioavailable drug products. The solubility of a drug, governed by the varying pH along the GI tract and its dissociation constant (pKa), is pivotal in determining its ionization state and absorption rate. Notably, weak acids and bases remain unionized and are absorbed more rapidly.
Enhanced drug absorption can be achieved by reducing particle sizes and increasing surface areas, thereby facilitating...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

First-in-human phase I/II, open-label study of mRNA-2416 alone or combined with durvalumab in patients with advanced solid tumors and ovarian cancer.

The oncologist·2025

Same author

Evaluation of single-cell sorting accuracy using antibody-derived tag-based qPCR.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2024

Same author

terraFlow, a high-parameter analysis tool, reveals T cell exhaustion and dysfunctional cytokine production in classical Hodgkin's lymphoma.

Cell reports·2024

Same author

Molecular cytometry for comprehensive immune profiling.

Methods in cell biology·2024

Same author

Co-staining with Fluorescent Antibodies and Antibody-Derived Tags for Cell Sorting Prior to CITE-Seq.

Methods in molecular biology (Clifton, N.J.)·2024

Same author

CD4 Phenotypes Are Associated with Reduced Expansion of Tumor-Infiltrating Lymphocytes in Melanoma Patients Treated with Adoptive Cell Therapy.

Journal of immunology (Baltimore, Md. : 1950)·2023

Search research articles

Related Experiment Video

Updated: Jan 30, 2026

Transcriptome Analysis of Single Cells

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

High-Parameter Single-Cell Analysis.

Pratip K Chattopadhyay¹, Aidan F Winters¹, Woodrow E Lomas¹

¹Precision Immunology Laboratory, Perlmutter Cancer Center, NYU Langone Health, New York, NY 10016, USA;

Annual Review of Analytical Chemistry (Palo Alto, Calif.)

|January 31, 2019

Summary

High-parameter single-cell technologies like flow cytometry and mass cytometry offer powerful biomarker discovery. These methods enable detailed cell type analysis and advance our understanding of cell biology and disease mechanisms.

Keywords:

CyTOF flow cytometry high-parameter molecular cytometry single-cell

More Related Videos

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Published on: June 14, 2016

Single-cell Microinjection for Cell Communication Analysis

Single-cell Microinjection for Cell Communication Analysis

Published on: February 26, 2017

Related Experiment Videos

Last Updated: Jan 30, 2026

Transcriptome Analysis of Single Cells

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Published on: June 14, 2016

Single-cell Microinjection for Cell Communication Analysis

Single-cell Microinjection for Cell Communication Analysis

Published on: February 26, 2017

Area of Science:

Biotechnology
Molecular Biology
Cell Biology

Background:

Thousands of transcripts and proteins define cell function and type.
High-parameter technologies enable simultaneous measurement of numerous cellular markers.
Cell-by-cell analysis is crucial for understanding cellular heterogeneity.

Purpose of the Study:

Review three key high-parameter single-cell technologies.
Highlight their potential for biomarker discovery and disease mechanism elucidation.
Provide an overview of high-parameter single-cell data analysis.

Main Methods:

Discuss high-parameter flow cytometry.
Describe mass cytometry.
Introduce integrated molecular cytometry platforms.

Main Results:

Detail hardware, instrumentation, and reagents for each platform.
Analyze the limitations and advantages of each technology.
Provide an overview of single-cell data analysis tools and processes.

Conclusions:

High-parameter single-cell technologies are vital for advancing cell biology.
These platforms facilitate biomarker discovery and understanding of disease.
Emerging data analysis methods are essential for interpreting complex single-cell data.