First Report of Grapevine redglobe virus (GRGV) in Grapevine in France
M Beuve1, T Candresse2, M Tannières1
1Institut National de la Recherche Agronomique, Université de Strasbourg, UMR 1131 santé de la Vigne et Qualité du Vin, 68021 Colmar Cedex, France.
Plant Disease
|February 1, 2019
Summary
Grapevine redglobe virus (GRGV) was detected in French Cabernet franc grapevines, marking its first report in France. Further research is needed to understand its prevalence and impact on the grapevine industry.
Area of Science:
- Plant Virology
- Grapevine Pathology
- Molecular Diagnostics
Background:
- Grapevine redglobe virus (GRGV), a Maculavirus, was first identified in Italy in 2000 and later in Greece and California.
- GRGV is disseminated through propagation and grafting, with no known vectors or seed transmission.
- GRGV infections are typically asymptomatic.
Purpose of the Study:
- To report the first detection of GRGV in France.
- To investigate the presence of GRGV in Vitis vinifera cv. Cabernet franc (CF) in Bordeaux.
- To analyze the genetic relatedness of the French GRGV isolate to other known isolates.
Main Methods:
- Next-generation sequencing (Illumina) of total RNAs from a symptomatic Cabernet franc vine.
- RT-PCR using specific primers designed from aligned GRGV sequences.
- Direct sequencing of RT-PCR amplicons.
Main Results:
- GRGV was identified in a Cabernet franc plant in Bordeaux, France, exhibiting fanleaf-like degeneration symptoms.
- RT-PCR analysis confirmed GRGV presence in 83% of tested Cabernet franc plants from the same plot.
- Sequenced amplicons showed high nucleotide identity (91.5-91.7%) to a reference GRGV sequence.
Conclusions:
- This study represents the first report of GRGV in France.
- The prevalence and potential threat of GRGV to the French grapevine industry require further investigation.
- Additional studies are needed to assess GRGV pathogenicity and its impact on grapevine vigor, rooting, and graft compatibility.
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