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Updated: Jan 30, 2026

In Situ Hybridization for the Precise Localization of Transcripts in Plants
Published on: November 23, 2011
In Situ Localization of Small RNAs in Plants
Cristina F Marco1, Damianos S Skopelitis1, Marja C P Timmermans2,3
1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA.
Abstract:
Small RNAs have vital roles in numerous aspects of plant biology. Deciphering their precise contributions requires knowledge of a small RNA's spatiotemporal pattern of accumulation. The in situ hybridization protocol described here takes advantage of locked nucleic acid (LNA) oligonucleotide probes to visualize small RNA expression at the cellular level with high sensitivity and specificity. The procedure is optimized for paraffin-embedded plant tissue sections, is applicable to a wide range of plants and tissues, and can be completed within 2-6 days.
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