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[Sequencing of a large off-ladder allele at Penta E locus]
Ying'ai Zhang1, Shunlan Wang, Xiaohong Wen
1Central Laboratory, Haikou People's Hospital, Haikou, Hainan 570208, China. zhangyingai@hotmail.com.
Objective:
To identify a rare large off ladder (OL) allele at the Penta E locus.
Methods:
Chelex-100 was used to extract DNAs from the blood samples. The PCR fragments were purified, extracted, and subjected to TA cloning and sequencing.
Results:
An OL allele was identified by the PowerPlex™ 21 at the Penta E locus, which was postulated as allele 26 based on assigned size. The OL allele was verified as a novel fragment containing 26 full AAAGA repeats.
Conclusion:
OL alleles and microvariants should be verified by direct sequencing. Typing of OL alleles has significance for both daily work and forensic genetics.
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