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Related Experiment Video

Updated: Jan 30, 2026

A High-throughput Calcium-flux Assay to Study NMDA-receptors with Sensitivity to Glycine/D-serine and Glutamate

A High-throughput Calcium-flux Assay to Study NMDA-receptors with Sensitivity to Glycine/D-serine and Glutamate

Published on: July 10, 2018

Locus-Specific DNA Methylation Assays to Study Glutamate Receptor Regulation.

Jordan A Brown¹, J David Sweatt¹, Garrett A Kaas²

¹Department of Pharmacology, Vanderbilt University, Nashville, TN, USA.

Methods in Molecular Biology (Clifton, N.J.)

|February 2, 2019

Summary

Epigenetic regulation of glutamate receptors is crucial for nervous system function. This study details two accessible methods, bisulfite sequencing and methyl-binding domain capture, for quantifying DNA methylation patterns in neurological research.

Keywords:

AMPA Bisulfite CpG DNA methylation Epigenetics Glutamate receptors MBDCap NMDA

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Last Updated: Jan 30, 2026

A High-throughput Calcium-flux Assay to Study NMDA-receptors with Sensitivity to Glycine/D-serine and Glutamate

A High-throughput Calcium-flux Assay to Study NMDA-receptors with Sensitivity to Glycine/D-serine and Glutamate

Published on: July 10, 2018

In vitro Methylation Assay to Study Protein Arginine Methylation

In vitro Methylation Assay to Study Protein Arginine Methylation

Published on: October 5, 2014

Methylated DNA Immunoprecipitation

Methylated DNA Immunoprecipitation

Published on: January 2, 2009

Area of Science:

Neuroscience
Epigenetics
Molecular Biology

Background:

Glutamate receptors are epigenetically regulated via histone modifications and DNA methylation.
Dysregulation of these epigenetic marks impacts glutamate receptor function in neurological diseases.
Epigenetic mechanisms are vital for nervous system function, necessitating robust quantification tools.

Purpose of the Study:

To describe and compare two widely used methods for quantifying DNA methylation.
To provide accessible techniques for researchers studying epigenetic regulation in the nervous system.

Main Methods:

Bisulfite sequencing: Modifies unmethylated cytosines to uracil, followed by PCR and sequencing to quantify methylation at the nucleotide level.
Methyl-binding domain capture (MBDCap): Enriches methylated DNA fragments using MBD peptides, followed by qRT-PCR for regional quantification.

Main Results:

Both methods allow for the quantification of DNA methylation at gene, regulatory region, or nucleotide levels.
These techniques are accessible, requiring common laboratory equipment and minimal specialized knowledge.

Conclusions:

Bisulfite sequencing and MBDCap are valuable tools for interrogating DNA methylation patterns.
These methods facilitate research into the role of epigenetics in neurological function and disease.