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Updated: Jan 30, 2026

Fluorimetric Techniques for the Assessment of Sperm Membranes
Published on: November 28, 2018
First birth of a healthy infant following intra-cytoplasmic sperm injection using a new permeable cryoprotectant-free
Llanos Medrano1, María Enciso2, María José Gomez-Torres3
1IVF Spain Alicante, Av. de Ansaldo, 13, 03540, Alicante, Spain.
Purpose:
The purpose of this study is to present the first birth of healthy infant born following ICSI using the new permeable cryoprotectant-free sperm vitrification protocol Easy-Sperm®.
Principal Results:
A 39 years old woman and his 40 years old partner underwent egg donation treatment at IVF-Spain Alicante (Spain). Half of the mature oocytes obtained from a young and healthy donor were fertilized by ICSI, using slow-frozen spermatozoa and the other half with vitrified spermatozoa. A total of 5 blastocysts were obtained on day 5 (3 resulting from vitrified spermatozoa and 2 from frozen sperm). The best embryo, with AA quality (derived from one of the oocytes fertilized with vitrified sperm) was transferred. The woman conceived and, following a normal pregnancy, delivered a healthy boy.
Conclusions:
To the best of our knowledge, this is the first case report of a successful pregnancy and delivery of a healthy infant from ICSI with permeable vitrified spermatozoa in an oocyte donation program with transfer on blastocyst stage.
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