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Expression and Characterization of MICU2, a Ca2+ Sensor Protein

Wenping Wu1, Jimin Zheng2, Zongchao Jia3

  • 1College of Chemistry, Beijing Normal University, Beijing, China.

Methods in Molecular Biology (Clifton, N.J.)
|February 3, 2019
PubMed
Summary

Mitochondrial calcium uptake regulator 2 (MICU2) protein preparation methods are detailed. These methods allow for in vitro characterization of MICU2, including its interaction with MICU1 and oligomerization.

Keywords:
Ca2+ sensorEF handMALLSMICU2MitochondrialPull-downPurificationSEC

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