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A flyover style microfluidic chip for highly purified magnetic cell separation
Shujing Lin1, Xiao Zhi2, Di Chen1
1School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, PR China; Shanghai Engineering Research Center for Intelligent Diagnosis and Treatment Instrument, Shanghai 200240, PR China; Key Lab. for Thin Film and Microfabrication Technology of Ministry of Education, Shanghai 200240, PR China.
Biosensors & Bioelectronics
|February 3, 2019
Summary
This study introduces a novel microfluidic chip for high-purity white blood cell (WBC) sorting using magnetic separation. The technology achieves excellent purity and viability, offering a promising tool for cancer diagnostics and biomedical applications.
Area of Science:
- Biomedical Engineering
- Cell Separation Technology
- Cancer Biomarkers
Background:
- White blood cells (WBCs) are crucial biomarkers for cancer diagnosis, treatment, and prognosis.
- Current methods for isolating high-purity WBCs, including microfluidic technologies, face significant challenges.
- Magnetic activated cell sorting (MACS) is a widely used clinical method, suggesting potential for integrated microfluidic-magnetic approaches.
Purpose of the Study:
- To develop and demonstrate a novel microfluidic chip for efficient and high-purity sorting of white blood cells (WBCs).
- To combine microfluidic principles with magnetic cell separation for enhanced WBC isolation.
- To evaluate the purity and viability of WBCs sorted using the developed system.
Main Methods:
- A unique flyover-style microfluidic chip was designed and fabricated.
- Two-stage magnetic separation utilizing lateral and vertical magnetic forces was employed for continuous flow WBC sorting.
- Immunomagnetic micro/nano-particles (IMNPs) were used to label WBCs for magnetic separation.
Main Results:
- The microfluidic chip achieved a final WBC separation purity of 93% ± 1.67% at a flow rate of 20 μL/min.
- The viability of the sorted WBCs was maintained at a high level of 97.5% ± 1.8%.
- The system demonstrated effective separation of WBCs using sequential magnetic forces.
Conclusions:
- The novel flyover microfluidic chip with integrated magnetic separation is a highly effective method for high-purity WBC sorting.
- This technology shows great potential for broad clinical application in cancer diagnostics and other biomedical fields.
- The system's design is adaptable for sorting other cell types, highlighting its versatility.
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