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High miR-3687 Expression Affects Migratory and Invasive Ability of Oesophageal Carcinoma
Kotaro Hagio1, Shinji Furuya2, Jiro Nakamura3
1First Department of Surgery, Faculty of Medicine University of Yamanashi, Yamanashi, Japan.
Background/Aim:
Basaloid squamous cell carcinoma of the oesophagus (BSCCE) is a variant of oesophageal squamous cell carcinoma (ESCC), that has a more aggressive biological behaviour than that of typical ESCC. miR-3687 has been previously identified to be highly expressed in BSCCE. This study aimed to evaluate the prognostic impact of miR-3687 in ESCC and determine the role of miR-3687 in ESCC motility.
Materials And Methods:
miR-3687 expression in human ESCC cell lines and in primary tumour samples obtained from patients with ESCC who underwent esophagectomy were analyzed via real-time polymerase chain reaction (PCR). Knockdown and over-expression experiments were conducted with miR-3687 siRNA and miRNA mimic, and the effect on cell proliferation, migration, and invasion was analyzed.
Results:
A total of 92 samples were analyzed. High miR-3687 expression was correlated with poor prognosis. miR-3687 upregulation promoted cell migration and invasion.
Conclusion:
miR-3687 expression negatively impacts the prognosis of patients with ESCC.
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