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Updated: Jan 29, 2026
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Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development
Published on: October 30, 2013
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Differential expression of S1P receptor subtypes in human bladder transitional cell carcinoma
A Palangi1, N Shakhssalim2, M Parvin3
1Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Summary
This study found that Sphingosine 1-phosphate receptors (S1PRs) 1, 2, and 3 are overexpressed in bladder tumors. Their expression profile in tumor biopsies may serve as a promising diagnostic marker for bladder cancer.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Sphingosine 1-phosphate (S1P) and its receptors (S1PRs) are implicated in cancer cell fate.
- S1PR subtype expression can vary with tumor type, stage, and pathology.
- Understanding S1PR expression in bladder cancer is crucial for potential diagnostic applications.
Purpose of the Study:
- To investigate the relationship between plasma S1P levels and S1PR subtype expression in bladder tumors.
- To evaluate S1PR expression in relation to tumor grade and stage.
- To determine if S1PR profiles in tumor biopsies can serve as diagnostic markers for bladder carcinoma.
Main Methods:
- Analysis of tumor biopsies and normal bladder tissues from 41 bladder cancer patients and 26 controls.
- Immunohistochemistry (IHC) and quantitative real-time RT-PCR were used to assess S1PR subtype expression.
- Plasma S1P levels were quantified using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
Main Results:
- Tissue S1PR1, S1PR2, and S1PR3 were significantly overexpressed in bladder tumors (3–104 fold increase).
- IHC confirmed the accumulation of S1PR subtypes 1 and 2 in tumor tissues.
- Plasma S1P levels in patients were comparable to control levels, suggesting localized tumor expression is key.
Conclusions:
- Overexpression of S1PR1, S1PR2, and S1PR3 in bladder tumor biopsies is evident.
- The S1PR expression profile correlates with pathological grades and stages of bladder cancer.
- S1PR profiles in tumor biopsies represent a promising biomarker for bladder carcinoma diagnosis.
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