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Human insulin gene in transgenic mouse lines.
M Fromont-Racine1, D Bucchini, P Desbois
1Institut Jacques Monod du CNRS, Unité INSERM 257, Université Paris VII, France.
Biomedica Biochimica Acta
|January 1, 1988
Summary
Researchers created transgenic mice with the human insulin gene. These mice express human C-peptide in their blood and urine, confirming the gene
Area of Science:
- Molecular Biology
- Genetics
- Endocrinology
Background:
- The regulation of human insulin gene expression is complex.
- Understanding the genetic elements controlling insulin production is crucial for diabetes research.
Purpose of the Study:
- To generate transgenic mouse models expressing the human insulin gene.
- To identify the regulatory sequences within the human insulin gene responsible for its correct expression.
Main Methods:
- Construction of a human insulin gene transgene with flanking regulatory regions (4 kb 5' and 5.5 kb 3').
- Generation of transgenic mouse lines carrying this construct.
- Detection of human C-peptide in serum and urine.
- Analysis of human insulin gene transcripts in pancreatic RNA.
Main Results:
- Successful generation of transgenic mouse lines harboring the human insulin gene.
- Detection of human C-peptide in both serum and urine of the transgenic mice.
- Evidence of specific human insulin gene transcripts in pancreatic RNA from these mice.
Conclusions:
- The introduced 5' and 3' flanking DNA sequences contain the necessary elements for proper phenotypic expression of the human insulin gene.
- These transgenic mice serve as a valuable model for studying human insulin gene regulation and function.