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Published on: April 6, 2017
Design, Synthesis, and Cellular Uptake of Oligonucleotides Bearing Glutathione-Labile Protecting Groups
Hisao Saneyoshi1, Takayuki Ohta1, Yuki Hiyoshi1
1Department of Material and Life Chemistry, Faculty of Engineering , Kanagawa University , 3-27-1 Rokkakubashi , Kanagawa-ku , Yokohama 221-8686 , Japan.
Abstract:
Glutathione-labile protecting groups for phosphodiester moieties in oligonucleotides were designed, synthesized, and incorporated into oligonucleotides. The protecting groups on the phosphodiester moieties were cleaved in a buffer containing 10 mM glutathione, which was used as a model of intracellular fluid. Cellular uptake of oligonucleotides bearing glutathione-labile protecting groups was strongly affected by the location and number of the protecting groups.
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