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Immunoaffinity isolation of a sialyl-Le(a) oligosaccharide from human milk
H Kitagawa1, H Nakada, Y Numata
1Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University.
Journal of Biochemistry
|October 1, 1988
Abstract:
A cancer-associated antigen, sialyl-Le(a) oligosaccharide, was isolated from human milk using a monoclonal antibody recognizing carbohydrate moieties of mucin-type glycoproteins. The structure was identified as: (Formula: see text) based on 500-MHz 1H-NMR spectroscopy. This oligosaccharide comprises 0.07% of sialyloligosaccharides in human milk. The NMR spectra of two fellow oligosaccharides, Le(a) oligosaccharide (or lacto-N-fucopentaose II) and LS-tetrasaccharide a, are also given.