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Characterization of a metazoan ADA acetyltransferase complex
Jelly H M Soffers1, Xuanying Li1, Anita Saraf1
1Stowers Institute for Medical Research, Kansas City, MO 64110, USA.
Nucleic Acids Research
|February 5, 2019
Summary
Researchers identified a new metazoan
Area of Science:
- * Molecular Biology
- * Biochemistry
- * Genomics
Background:
- * Gcn5 acetyltransferase is a key enzyme in yeast and metazoan complexes.
- * Yeast has a small ADA complex, but a metazoan equivalent was unknown.
- * Drosophila studies suggested a small Gcn5-containing complex.
Purpose of the Study:
- * To purify and characterize a novel metazoan complex containing Gcn5.
- * To determine the composition and function of this newly identified complex.
- * To investigate the relationship between this complex and the SAGA complex.
Main Methods:
- * Protein complex purification from Drosophila.
- * Mass spectrometry for composition analysis.
- * In vitro histone acetylation assays.
- * Chromatin immunoprecipitation sequencing (ChIP-Seq).
Main Results:
- * A new metazoan complex, named 'ADA complex', was purified and found to contain Gcn5, Ada2b, Ada3, and Sgf29.
- * The fly ADA complex exhibits histone acetylation activity on histones and nucleosomes.
- * ChIP-Seq revealed Ada2b binding sites, some overlapping with Spt3 (SAGA subunit) and others independent of SAGA.
Conclusions:
- * A metazoan ADA complex analogous to the yeast ADA complex exists.
- * This complex possesses histone acetyltransferase activity.
- * The ADA complex can bind chromatin independently of the larger SAGA complex, suggesting distinct roles.
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