Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Manipulation and Analysis01:21

Manipulation and Analysis

299
GIS manipulation and analysis functions are vital for decision-making and planning. These activities range from data retrieval tasks, such as selecting information based on specific criteria, to advanced analytical techniques that address complex spatial problems.One critical GIS analysis method is overlaying, which combines multiple data layers to examine impacts. For example, overlaying a river-dammed lake boundary with road networks can identify affected infrastructure. Another common...
299
Clinical Applications of Epidermal Stem Cells01:19

Clinical Applications of Epidermal Stem Cells

3.3K
Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...
3.3K
Therapeutic Drug Monitoring: Drug Analysis Methods01:26

Therapeutic Drug Monitoring: Drug Analysis Methods

192
Therapeutic Drug Monitoring (TDM) is a clinical practice that measures specific drug levels in a patient's blood or body tissues to tailor drug therapy effectively. This monitoring is critical for managing drugs with narrow therapeutic indices like digoxin and phenytoin, ensuring they are both safe and effective. For instance, monitoring theophylline levels in asthma patients involves precision and sensitivity to adjust doses according to individual responses to therapy, ensuring efficacy and...
192
Parametric Survival Analysis: Weibull and Exponential Methods01:14

Parametric Survival Analysis: Weibull and Exponential Methods

1.1K
Parametric survival analysis models survival data by assuming a specific probability distribution for the time until an event occurs. The Weibull and exponential distributions are two of the most commonly used methods in this context, due to their versatility and relatively straightforward application.
Weibull Distribution
The Weibull distribution is a flexible model used in parametric survival analysis. It can handle both increasing and decreasing hazard rates, depending on its shape parameter...
1.1K
Cancers Originate from Somatic Mutations in a Single Cell02:21

Cancers Originate from Somatic Mutations in a Single Cell

14.9K
Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
14.9K
Photoluminescence: Applications01:14

Photoluminescence: Applications

1.1K
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adaptative two-phase thermal circulation system for complex-shaped electronic device cooling.

Nature communications·2025
Same author

Fabrication of Hierarchical Microstructures via Laser-Induced Shrinkage of Shape Memory Polymers for Flexible Pressure Sensing.

ACS applied materials & interfaces·2024
Same author

Laser Fabrication of Humidity Sensors on Ethanol-Soaked Polyimide for Fully Contactless Respiratory Monitoring.

ACS applied materials & interfaces·2024
Same author

Facile and Cost-Effective Fabrication of Highly Sensitive, Fast-Response Flexible Humidity Sensors Enabled by Laser-Induced Graphene.

ACS applied materials & interfaces·2023
Same author

Integrating In-Plane Thermoelectricity and Out-Plane Piezoresistivity for Fully Decoupled Temperature-Pressure Sensing.

Small (Weinheim an der Bergstrasse, Germany)·2023
Same author

Nonlinearity synergy: An elegant strategy for realizing high-sensitivity and wide-linear-range pressure sensing.

Nature communications·2023
Same journal

Correction: Kang et al. Fluid Flow to Electricity: Capturing Flow-Induced Vibrations with Micro-Electromechanical-System-Based Piezoelectric Energy Harvester. <i>Micromachines</i> 2024, <i>15</i>, 581.

Micromachines·2026
Same journal

Femtosecond Laser Texturing of Wood Coatings with Bio-Based Epoxy and Wax Additives for Enhanced Hydrophobicity.

Micromachines·2026
Same journal

Engineering of Optoelectronic Devices for Renewable Energy Applications.

Micromachines·2026
Same journal

Phase Transformation and Electrochemical Behavior of Hexagonal TiO<sub>2</sub> Nanotubes Under Different Annealing Temperatures and Heating Rates.

Micromachines·2026
Same journal

Process Optimization and Predictive Modeling of Femtosecond Laser Precision Milling for Commercial PMMA Slices.

Micromachines·2026
Same journal

A Hybrid Preprocessing Multi-Objective Surrogate Model for Thermal MEMS Actuators.

Micromachines·2026
See all related articles

Related Experiment Video

Updated: Jan 29, 2026

Single-cell Microfluidic Analysis of Bacillus subtilis
10:37

Single-cell Microfluidic Analysis of Bacillus subtilis

Published on: January 26, 2018

12.6K

Microfluidic Single-Cell Manipulation and Analysis: Methods and Applications.

Tao Luo1, Lei Fan2, Rong Zhu3

  • 1Department of Biomedical Engineering, City University of Hong Kong, Hong Kong, China. taoluo4-c@my.cityu.edu.hk.

Micromachines
|February 6, 2019
PubMed
Summary
This summary is machine-generated.

Microfluidics enables precise single-cell analysis, overcoming limitations of traditional methods. This technology is crucial for understanding cell heterogeneity in disease diagnostics and therapeutics.

Keywords:
microfluidicssingle-cell analysissingle-cell manipulation

More Related Videos

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

15.5K
Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
12:04

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Published on: December 6, 2013

12.9K

Related Experiment Videos

Last Updated: Jan 29, 2026

Single-cell Microfluidic Analysis of Bacillus subtilis
10:37

Single-cell Microfluidic Analysis of Bacillus subtilis

Published on: January 26, 2018

12.6K
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

15.5K
Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
12:04

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Published on: December 6, 2013

12.9K

Area of Science:

  • Biotechnology
  • Cell Biology
  • Microfluidics

Background:

  • Cellular heterogeneity is critical for disease diagnostics and therapeutics but often masked by population-level studies.
  • Traditional methods struggle with manipulating and analyzing small, low-concentration single cells.
  • Microfluidics offers advanced solutions for single-cell manipulation and analysis.

Purpose of the Study:

  • To review advancements in microfluidic technologies for single-cell manipulation and analysis.
  • To highlight the methods and applications of microfluidics in single-cell research.
  • To discuss the pros and cons of microfluidic approaches and future trends.

Main Methods:

  • Review of microfluidic techniques including hydrodynamic and electrical manipulation.
  • Summary of single-cell analysis at cellular and genetic levels using microfluidics.
  • Discussion of advantages and disadvantages of various microfluidic methods.

Main Results:

  • Microfluidics provides higher throughput, smaller sample volumes, and reduced contamination risk compared to conventional methods.
  • Diverse microfluidic methods enable precise manipulation and comprehensive analysis of single cells.
  • Significant progress has been made in applying microfluidics to single-cell studies over the past two decades.

Conclusions:

  • Microfluidics is an ideal technology for statistically meaningful single-cell research.
  • The review summarizes key methods and applications, offering insights into future trends.
  • Microfluidic single-cell analysis is vital for advancing our understanding of cellular heterogeneity.