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Identification of the targets of hematoporphyrin derivative in lung adenocarcinoma using integrated network analysis

Hongtao Yin1, Yan Yu2

  • 1Department of Radiation Oncology, Harbin Medical University Cancer Hospital, Harbin, 150081, Heilongjiang, China.

Biological Research
|February 6, 2019
PubMed
Abstract

Insights

Hematoporphyrin derivative (HPD) sensitizes lung adenocarcinoma. This study identified potential HPD target genes, including RPL3, HSP90AA1, ATAD2, PIAS1, and USP25, offering insights into treatment mechanisms.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genomics

Background:

  • Hematoporphyrin derivative (HPD) exhibits a photosensitizing effect in lung adenocarcinoma.
  • Understanding HPD's molecular targets is crucial for optimizing lung adenocarcinoma treatment.

Purpose of the Study:

  • To identify target genes regulated by Hematoporphyrin derivative (HPD) in lung adenocarcinoma.
  • To elucidate the molecular mechanisms underlying HPD's therapeutic effects.

Main Methods:

  • RNA sequencing of A549 lung adenocarcinoma cells under various treatment conditions (untreated, X-ray, X-ray + HPD).
  • Differential gene expression analysis using Mfuzz, enrichment analysis, and protein-protein interaction network construction with Cytoscape.
  • Prediction of microRNA and transcription factor targets using WebGestalt and ITFP, followed by integrated network visualization.

Main Results:

  • Identified 815 differentially expressed genes (DEGs) in G1 and 464 DEGs in G2.
  • Discovered interactions between ribosomal protein L3 (RPL3) and heat shock protein 90 kDa alpha, class A member 1 (HSP90AA1) in G1.
  • Predicted transcription factors AAA domain containing 2 (ATAD2) and protein inhibitor of activated STAT 1 (PIAS1) for G1 and G2, respectively. Ubiquitin-specific peptidase 25 (USP25) was found to be targeted by miR-200b, miR-200c, and miR-429 in G2.

Conclusions:

  • Ribosomal protein L3 (RPL3), heat shock protein 90 kDa alpha, class A member 1 (HSP90AA1), AAA domain containing 2 (ATAD2), and protein inhibitor of activated STAT 1 (PIAS1) are potential HPD targets.
  • Ubiquitin-specific peptidase 25 (USP25), targeted by miR-200b, miR-200c, and miR-429, is another potential HPD target in lung adenocarcinoma.
  • These findings provide insights into the molecular mechanisms of HPD in lung adenocarcinoma treatment.
Keywords:
Hematoporphyrin derivativeIntegrated networkLung adenocarcinomaProtein–protein interaction networkX-ray

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