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Long non-coding RNA XIST regulates miR-106b-5p/P21 axis to suppress tumor progression in renal cell carcinoma

Ke Sun1, Zhankui Jia2, Ranran Duan3

  • 1Department of Urology, The First Affiliated Hospital, Zhengzhou University, No 1 Jianshe East Rd., Zhengzhou, 450052, People's Republic of China; Urological Institute of Henan, Zhengzhou, 450052, Henan Province, People's Republic of China.

Biochemical and Biophysical Research Communications
|February 6, 2019
PubMed

Insights

Long non-coding RNA XIST is downregulated in renal cell carcinoma (RCC). Upregulating XIST inhibits RCC progression by regulating the miR-106b-5p/P21 axis, suggesting therapeutic potential.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are critical in cancer.
  • The role of lncRNA XIST in renal cell carcinoma (RCC) is unclear.

Purpose of the Study:

  • Investigate XIST's function and mechanism in RCC.
  • Explore XIST's potential as a therapeutic target.

Main Methods:

  • Quantitative real-time PCR to assess XIST expression.
  • In vitro and in vivo assays to evaluate XIST's functional impact.
  • MiRNA interaction analysis (e.g., luciferase assay) to elucidate mechanisms.

Main Results:

  • XIST expression is reduced in RCC tissues and cells.
  • XIST overexpression suppresses RCC cell proliferation and induces G0/G1 arrest.
  • XIST directly targets miR-106b-5p, upregulating P21 expression.
  • Curcumin affects the XIST/miR-106b-5p/P21 pathway in RCC cells.

Conclusions:

  • XIST acts as a tumor suppressor in RCC by sponging miR-106b-5p and regulating P21.
  • The XIST/miR-106b-5p/P21 axis presents a novel therapeutic strategy for RCC.
  • XIST holds promise for RCC treatment and warrants further investigation.
Keywords:
Cell proliferationCurcuminP21XISTmiR-106b-5p

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