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MiR-127-3p targets KIF3B to inhibit the development of oral squamous cell carcinoma

L Ji1, Z-N Zhu, C-J He

  • 1Department of General Dentistry, The Affiliated Stomatological Hospital of Nanchang University, Nanchang, China. sdlcjl516@126.com.

European Review for Medical and Pharmacological Sciences
|February 6, 2019
PubMed
Abstract

Insights

MicroRNA-127-3p (miR-127-3p) is down-regulated in oral squamous cell carcinoma (OSCC). Restoring miR-127-3p inhibits OSCC progression by targeting KIF3B, offering a potential therapeutic strategy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) are implicated in cancer development.
  • Oral squamous cell carcinoma (OSCC) pathogenesis involves dysregulated gene expression.
  • The specific role of microRNA-127-3p (miR-127-3p) in OSCC requires elucidation.

Purpose of the Study:

  • To investigate the function of miR-127-3p in oral squamous cell carcinoma (OSCC).
  • To identify the molecular targets and mechanisms underlying miR-127-3p's role in OSCC.

Main Methods:

  • Quantitative Real-time polymerase chain reaction (qRT-PCR) and Western blot to assess miR-127-3p and KIF3B expression in OSCC.
  • MTT and transwell assays to evaluate the functional impact of miR-127-3p and KIF3B on OSCC cells.
  • Luciferase reporter assay to confirm the direct targeting of KIF3B by miR-127-3p.

Main Results:

  • Down-regulation of miR-127-3p was observed in OSCC tissues, correlating with adverse clinicopathological features and poor prognosis.
  • Overexpression of miR-127-3p suppressed OSCC cell proliferation, migration, and invasion.
  • KIF3B was identified as a direct target of miR-127-3p and was found to be upregulated in OSCC, promoting tumorigenesis.
  • Upregulation of KIF3B partially counteracted the inhibitory effects of miR-127-3p in OSCC development.

Conclusions:

  • MiR-127-3p acts as a tumor suppressor in OSCC by targeting KIF3B.
  • The miR-127-3p/KIF3B axis regulates OSCC cell proliferation, migration, and invasion.
  • Modulating miR-127-3p presents a potential therapeutic avenue for OSCC treatment.

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