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On-line fast atom bombardment analysis of dynamic biological systems
1Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston 77030.
Biomedical & Environmental Mass Spectrometry
|October 1, 1988
Abstract:
The continuous-flow fast atom bombardment probe interface has been used for the direct analysis of aqueous biochemical reactions. This device was used in two ways: in a constant flow mode and a flow injection mode. Each method has particular advantages which are illustrated with specific applications, including measurement of enzyme kinetics, comparative rates of hydrolysis of multi-substrate/single enzyme reactions, C-terminal peptide sequence analysis, and characterization of multi-enzyme/single substrate systems.