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Updated: May 5, 2026

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
Published on: October 24, 2018
An integral probe for capillary zone electrophoresis/continuous-flow fast atom bombardment mass spectrometry
1The Analytical Chemistry Center and Department of Biochemistry and Molecular Biology, University of Texas Medical School, 77030, Houston, TX.
Abstract:
An integral probe for capillary zone electrophoresis/continuous-flow fast atom bombardment mass spectrometry was constructed and operated in either the coaxial or liquid-junction interface mode. Results using these interfaces for the analyses of synthetic peptides are presented. The coaxial arrangement attains the best electrophoretic performance, generally providing a greater number of theoretical plates. However, in that the electrophoresis times are generally greater for the liquid-junction interface because there is no mechanical flow resulting from the source vacuum, the overall separation efficiency of the liquid-junction interface is equal to or greater than that of the coaxial interface. In addition, the liquid-junction interface is easier to set up and operate, and allows larger inner diameter capillaries to be used to achieve higher sample loads.
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