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Updated: Jan 29, 2026

Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification

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Published on: November 28, 2016

Small Non-Coding RNAs Derived From Eukaryotic Ribosomal RNA.

Marine Lambert¹, Abderrahim Benmoussa¹, Patrick Provost²

¹CHU de Québec Research Center/CHUL Pavilion, 2705 Blvd Laurier, Quebec City, QC, G1V 4G2 and Department of Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Université Laval, Quebec City, QC G1V 0A6, Canada.

|February 6, 2019

Summary

This summary is machine-generated.

Small non-coding RNAs called ribosomal RNA fragments (rRFs) are emerging as important gene regulators. These molecules, derived from ribosomal RNA, were previously overlooked but are now gaining attention for their regulatory roles.

Keywords:

biogenesis microRNAs ribosomal DNA (rDNA)ribosomal RNA-derived fragment (rRF)ribosomes small RNAs small ribosomal RNA (srRNA)

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Area of Science:

Molecular Biology
Genomics
RNA Biology

Background:

RNA-sequencing (RNA-Seq) has expanded the knowledge of small non-coding RNAs (ncRNAs).
Many ncRNAs originate from the processing of longer RNA precursors.
Ribosomal RNA (rRNA) is a major source of these ncRNAs, yielding rRNA fragments (rRFs).

Purpose of the Study:

To present a list of experimentally verified rRNA fragments (rRFs).
To discuss the origin, biogenesis, and characteristics of rRFs.
To explore the potential roles of rRFs in gene expression regulation.

Main Methods:

Literature review of experimentally verified rRFs.
Analysis of rRFs' origin, length, structure, and biogenesis.
Investigation of rRFs' association with regulatory proteins.

Main Results:

rRFs are a class of small ncRNAs derived from eukaryotic rRNA.
Their investigation was historically limited due to exclusion from RNA-Seq datasets.
rRFs are increasingly recognized for their potential biological significance.

Conclusions:

rRFs represent a newly appreciated class of regulatory ncRNAs.
Further research is needed to fully understand their functions.
Challenging existing dogmas is crucial for scientific discovery, as exemplified by rRFs.