Related Experiment Video
Updated: Jan 29, 2026

Selective Capture of 5-hydroxymethylcytosine from Genomic DNA
Published on: October 5, 2012
Artificial Restriction DNA Cutter Using Nuclease S1 for Site-Selective Scission of Genomic DNA
Arivazhagan Rajendran1,2, Narumi Shigi2,3, Jun Sumaoka2,4
1Institute of Advanced Energy, Kyoto University, Kyoto, Japan.
Abstract:
By combining a pair of pseudo-complementary peptide nucleic acids (pcPNAs) with S1 nuclease, a novel tool to cut DNA at a predetermined site can be obtained. Complementary pcPNAs invade the DNA duplex and base pair to each strand of a target site, creating single-stranded regions that are cleaved by S1 nuclease. The scission site can be freely modulated by the design of pcPNAs. This method can be used to cleave a single site in the human genome. This protocol presents experimental details for site-selective scission using this versatile new tool. © 2019 by John Wiley & Sons, Inc.
Related Concept Videos
Genomic DNA in Prokaryotes
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Genomic DNA in Eukaryotes
DNA Helicases
DNA Topoisomerases
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
DNA-only Transposons
The donor site from where the transposon is excised is either degraded or...
Recombinant DNA

