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Up-regulation of the MicroRNA miR-30c Induced by High Mobility Group Box 1 in A549 Cells Used as an Alveolar

Fumihiro Nagayasu, Kazuhisa Konishi, Naoki Ijiri

    Osaka City Medical Journal
    |February 6, 2019
    PubMed
    Abstract

    Insights

    High mobility group box 1 (HMGB1) induces microRNA (miRNA) miR-30c up-regulation and apoptosis in lung cells, suggesting a role in chronic obstructive pulmonary disease (COPD) pathogenesis.

    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Pulmonary Medicine

    Background:

    • MicroRNAs (miRNAs) are implicated in various diseases, including chronic obstructive pulmonary disease (COPD).
    • Alveolar apoptosis is a potential factor in COPD development.
    • High mobility group box 1 (HMGB1) is a cytokine linked to COPD pathogenesis.

    Purpose of the Study:

    • To investigate the association between HMGB1 and miRNA expression.
    • To explore the role of miRNAs in HMGB1-induced cellular injury and apoptosis.
    • To establish a link between HMGB1, miR-30c, and alveolar apoptosis in COPD.

    Main Methods:

    • A549 lung cells were stimulated with recombinant HMGB1.
    • Gene and protein expression analyzed via RT-PCR, ELISA, and Western blotting.
    • Apoptosis assessed using TUNEL assay and caspase-7 protein levels.

    Main Results:

    • HMGB1 treatment upregulated TNF-α, MIP-2, and MMP-7.
    • miR-30c expression increased following HMGB1 stimulation.
    • HMGB1 induced cellular injury and apoptosis, evidenced by altered CCNA2, PTEN, and pro-caspase-7 levels.

    Conclusions:

    • HMGB1 stimulation leads to miR-30c upregulation and apoptosis in A549 cells.
    • This study models the potential involvement of HMGB1 and miR-30c in COPD-related alveolar apoptosis.
    • Findings suggest HMGB1 and miR-30c as targets for future COPD research.

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