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Self-Organization of Fluids in a Multienzymatic Pump System.
Subhabrata Maiti1, Oleg E Shklyaev2, Anna C Balazs2
1Department of Chemistry , The Pennsylvania State University , 104 Chemistry Building , University Park , Pennsylvania 16802 , United States.
Langmuir : the ACS Journal of Surfaces and Colloids
|February 6, 2019
Summary
Microscale flow chemistry uses enzyme-powered microchambers to create self-organizing fluidic circuits. This research enables autonomous fluid transport and advances the automation of microfluidic devices.
Area of Science:
- Chemistry
- Fluid Dynamics
- Biotechnology
Background:
- Microscale flow chemistry optimizes reactions in microchambers using flowing fluids.
- Catalytic reactions on microchamber surfaces can act as pumps, influencing fluid flow and vice versa.
Purpose of the Study:
- To understand the dynamic interplay between catalytic reactions and fluid flow in microscale systems.
- To design a controllable microfluidic system using enzyme-powered pumps.
Main Methods:
- Experimental and simulation-based design of a microchamber system.
- Immobilization of three different enzymes in separate gels on the microchamber surface.
- Utilizing a reaction cascade to control temporal interactions between enzyme pumps.
Main Results:
- Enzyme-filled gels act as pumps, propelling fluids within the microchamber.
- A triangular arrangement of three pumps creates coordinated spatio-temporal interactions, forming well-defined fluidic circuits.
- Circuit layout and flow direction are controllable via gel placement and catalyst type.
Conclusions:
- The study presents a novel route for generating self-organizing and bifurcating fluids.
- Findings offer fundamental insights into nonequilibrium dynamical systems.
- The research provides guidelines for automating microfluidic devices through internally generated flows.
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