Related Experiment Video
Updated: Jan 29, 2026

Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
Published on: June 26, 2010
A sensitive and selective fluorescence probe for detection of hypochlorite (OCl-) and its bioimaging in live cells
Zhiwei Ma1, Xiao Wang2, Chuanchuan Wang1
1Faculty of Science, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China.
Abstract:
A novel indolium-based fluorescent probe (probe 1) for the recognition and detection of hypochlorite (OCl-) has been explored via a double oxidation reaction mechanism. Probe 1 exhibited excellent selectivity and sensitivity for OCl- over other analytes, and with a detection limit of 0.11 μM. Meanwhile, probe 1 showed fast response toward OCl- in less than 3 min with obvious changes in color, which could be observed by naked eye. Moreover, fluorescence imaging experiments by using Eca109 cells were performed utilizing the new probe, demonstrating its practical applications in living cells.
Related Concept Videos
What is Natural Selection?
T Cell Activation and Clonal Selection
Naive T cells that have not yet encountered an antigen express two primary CD...
Antibiotic Selection
Protein Dynamics in Living Cells
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Types of Selection
Frequency-dependent Selection

