A Highly Selective "Turn-on" Fluorescent Probe for Detection of Fe³⁺ in Cells

A new "turn-on" fluorescent probe Py based on rhodamine and piperonaldehyde was designed and synthesized for detecting Fe³⁺ in cells. The free probe Py was non-fluorescent. While only upon addition of Fe³⁺, the significant increase of the fluorescence and color were observed which could be visible directly by "naked-eye". The probe Py shows high selectivity and sensitivity for Fe³⁺ over other common metal ions in EtOH-H₂O (3/2, v/v) mixed solution. The association constant and the detection limit were calculated to be 4.81 × 10⁴ M^-1 and 1.18 × 10^-8 mol/L respectively. The introduction of piperonaldehyde unit could increase probe rigidity which could enhance its optical properties. Meanwhile, the binding mode between Py and Fe³⁺ was found to be a 1:1 complex formation. The density functional theory (DFT) calculations were performed which would further confirm the recognition mechanism between probe Py and Fe³⁺. In addition, the probe has been proved to be reversible for detecting Fe³⁺. Moreover, the probe Py was used to detect Fe³⁺ in cells successfully.