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A multicenter comparison of MOG-IgG cell-based assays
Patrick J Waters1, Lars Komorowski1, Mark Woodhall1
1From the Oxford Autoimmune Neurology Group (P.J.W., M.W., S.R.I.), Nuffield Department of Clinical Neurosciences, UK; Institute for Experimental Immunology (L.K., S.L.), Affiliated to Euroimmun AG, Luebeck, Germany; and Departments of Neurology (M.M., E.P.F., A.C.K., A.M., S.J.P.) and Laboratory Medicine and Pathology (J.F., J.M., E.P.F., A.C.K., A.M., S.J.P.), Mayo Clinic, College of Medicine, Rochester, MN.
Neurology
|February 8, 2019
Summary
Three myelin oligodendrocyte glycoprotein-immunoglobulin G (IgG) cell-based assays (CBAs) show high agreement. Live cell assays offer more reliable results for MOG autoimmune disorders.
Area of Science:
- Neuroimmunology
- Autoimmune Disorders
- Diagnostic Assays
Background:
- Myelin oligodendrocyte glycoprotein-immunoglobulin G (MOG-IgG) is a key biomarker for certain autoimmune neurological disorders.
- Standardization of MOG-IgG detection methods is crucial for accurate diagnosis and patient management.
- Existing cell-based assays (CBAs) for MOG-IgG vary across different international centers.
Purpose of the Study:
- To compare the performance of three distinct MOG-IgG CBAs from different international laboratories.
- To assess the concordance and diagnostic reliability of fixed and live cell-based assay methodologies.
- To evaluate the clinical specificity and predictive values of these MOG-IgG assays.
Main Methods:
- Serum samples from 394 patients, including those with MOG-IgG-associated disorders and controls, were analyzed.
- Three CBAs were employed: Euroimmun fixed CBA, Oxford live cell CBA, and Mayo live cell fluorescence-activated cell sorting (FACS) assay.
- Seropositivity was determined by visual microscopy or flow cytometry.
Main Results:
- A high overall agreement was observed among the three MOG-IgG CBAs.
- Live cell-based assays demonstrated superior positive predictive values (PPVs) compared to the fixed cell assay.
- Clinical specificities ranged from 98.1% to 100%, and PPVs ranged from 82.1% to 100%.
Conclusions:
- The study confirms a high degree of concordance across different MOG-IgG CBAs.
- Live cell-based MOG-IgG assays are more reliable indicators of MOG autoimmune spectrum disorders due to their higher PPVs.
- These findings support the use of standardized live cell assays for improved diagnosis of MOG-IgG-associated conditions.
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