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Related Experiment Video

Updated: Jan 29, 2026

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
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Multiplex Viral Detection Platform Based on a Aptamers-Integrated Microfluidic Channel.

Nileshi Saraf1, Michael Villegas2, Bradley Jay Willenberg1

  • 1Advanced Materials Processing and Analysis Centre, Department of Materials Science and Engineering, Department of Internal Medicine, College of Medicine, and Nanoscience Technology Centre, University of Central Florida, Orlando, Florida 32827, United States.

ACS Omega
|February 8, 2019
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Summary

This study presents a novel microfluidic device for detecting Zika and chikungunya viral proteins. The aptamer-based system offers high sensitivity and specificity for early disease detection.

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Microfluidics

Background:

  • Viral infections like Zika and chikungunya pose significant public health challenges.
  • Accurate and rapid detection of viral envelope proteins is crucial for timely diagnosis and disease management.
  • Existing detection methods may lack the sensitivity, specificity, or multiplexing capabilities required for comprehensive diagnostics.

Purpose of the Study:

  • To develop a polydimethylsiloxane-based microfluidic device for the simultaneous detection of multiple viral envelope proteins.
  • To enhance detection sensitivity and specificity through aptamer-analyte interactions and microfluidic channel design.
  • To establish a colorimetric assay for visualizing and quantifying viral protein presence.

Main Methods:

  • Fabrication of a polydimethylsiloxane microfluidic device with integrated microsized pillars to increase surface area.
  • Functionalization of the microfluidic channel with aptamers for specific binding of viral envelope proteins.
  • Utilizing aptamer-functionalized gold nanoparticles (AuNPs) to form a sandwich complex with target proteins.
  • Developing a silver-based colorimetric reaction for signal amplification and visualization.

Main Results:

  • The microfluidic device demonstrated multiplex detection of Zika and chikungunya envelope proteins on a single platform.
  • The integrated pillars enhanced aptamer attachment, leading to increased system sensitivity.
  • The assay successfully detected clinically relevant concentrations of viral proteins in both buffer (1 pM) and blood (100 pM) samples.
  • High specificity was achieved using gold-decorated aptamers within the microfluidic channel.

Conclusions:

  • The developed microfluidic device offers a sensitive, specific, and multiplexed platform for detecting viral envelope proteins.
  • This technology holds potential for rapid point-of-care diagnostics for viral infections.
  • The aptamer-AuNP-microfluidic system represents a promising advancement in biosensing for infectious diseases.