Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Colonization of Land02:22

The Colonization of Land

37.6K
Changes in the environment of the early Earth drove the evolution of organisms. As prokaryotic organisms in the oceans began to photosynthesize, they produced oxygen. Eventually, oxygen saturated the oceans and entered the air, resulting in an increase in atmospheric oxygen concentration, known as the oxygen revolution approximately 2.3 billion years ago. Therefore, organisms that could use oxygen for cellular respiration had an advantage. More than 1.5 years ago, eukaryotic cells and...
37.6K
Bone Cells and Tissue01:30

Bone Cells and Tissue

8.3K
Bones contain a relatively small number of cells entrenched in a matrix of organic and inorganic components. Although bone cells compose only a small amount of the bone volume, they are crucial to its function. Four types of cells are found within the bone tissue— osteoblasts, osteocytes, osteogenic cells, and osteoclasts.
Osteoblasts and Osteocytes
The osteoblast is the bone cell responsible for forming new bone tissue. It is found in the growing portions of bone, including the...
8.3K
Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

3.3K
Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
3.3K
Adaptive Mechanisms in Cancer Cells02:53

Adaptive Mechanisms in Cancer Cells

7.1K
Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
7.1K
Bone Structure01:55

Bone Structure

51.7K
Within the skeletal system, the structure of a bone, or osseous tissue, can be exemplified in a long bone, like the femur, where there are two types of osseous tissue: cortical and cancellous.
51.7K
Bone Remodeling01:40

Bone Remodeling

40.4K
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
40.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Circulating Levels of Epirubicin Cause Endothelial Senescence While Compromising Metabolic Activity and Vascular Function.

Frontiers in cell and developmental biology·2020
Same author

The bone metastasis niche in breast cancer-potential overlap with the haematopoietic stem cell niche <i>in vivo</i>.

Journal of bone oncology·2019
Same author

Endogenous Production of IL1B by Breast Cancer Cells Drives Metastasis and Colonization of the Bone Microenvironment.

Clinical cancer research : an official journal of the American Association for Cancer Research·2019
Same author

Parathyroid Hormone (PTH) Increases Skeletal Tumour Growth and Alters Tumour Distribution in an In Vivo Model of Breast Cancer.

International journal of molecular sciences·2018
Same author

Zoledronic acid alters hematopoiesis and generates breast tumor-suppressive bone marrow cells.

Breast cancer research : BCR·2017
Same author

Confocal/two-photon microscopy in studying colonisation of cancer cells in bone using xenograft mouse models.

BoneKEy reports·2016

Related Experiment Video

Updated: Jan 29, 2026

Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow
08:41

Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow

Published on: July 28, 2016

8.7K

Tracking Cancer Cells Colonization in Rodent Bone Using Two-Photon Microscopy.

Gloria Allocca1, Ning Wang2

  • 1Department of Oncology and Metabolism, The Mellanby Centre for Bone Research, The University of Sheffield, Sheffield, UK.

Methods in Molecular Biology (Clifton, N.J.)
|February 8, 2019
PubMed
Summary

Two-photon microscopy enables detailed tracking of cancer cell colonization in bone. This protocol details its use with frozen bone samples from mouse models for cancer research.

Keywords:
BoneCancer metastasisTwo-photon microscopyXenograft

More Related Videos

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy
17:08

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

Published on: August 6, 2014

13.6K
In situ Imaging of the Mouse Thymus Using 2-Photon Microscopy
15:00

In situ Imaging of the Mouse Thymus Using 2-Photon Microscopy

Published on: January 25, 2008

12.0K

Related Experiment Videos

Last Updated: Jan 29, 2026

Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow
08:41

Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow

Published on: July 28, 2016

8.7K
In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy
17:08

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

Published on: August 6, 2014

13.6K
In situ Imaging of the Mouse Thymus Using 2-Photon Microscopy
15:00

In situ Imaging of the Mouse Thymus Using 2-Photon Microscopy

Published on: January 25, 2008

12.0K

Area of Science:

  • Biomedical imaging
  • Cancer metastasis research
  • Skeletal biology

Background:

  • Two-photon microscopy is a valuable technique for bone research, offering qualitative and quantitative data.
  • Understanding cancer cell homing to bone is crucial for developing effective treatments.

Purpose of the Study:

  • To provide a detailed protocol for tracking cancer cell colonization in bone using two-photon microscopy.
  • To demonstrate the application of this technique in xenograft mouse models.

Main Methods:

  • Utilizing two-photon microscopy on frozen bone samples.
  • Employing xenograft mouse models with cancer cell implantation.
  • Step-by-step procedural description for imaging and analysis.

Main Results:

  • Successful visualization and tracking of cancer cell colonization patterns within bone structures.
  • Quantitative assessment of cancer cell distribution and density.
  • Demonstration of the technique's efficacy in preclinical cancer models.

Conclusions:

  • Two-photon microscopy provides a robust method for studying bone metastasis.
  • The described protocol facilitates detailed investigation of cancer cell-bone interactions.
  • This technique can aid in evaluating therapeutic strategies targeting bone metastasis.