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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

1.7K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
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Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
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Bone Structure01:55

Bone Structure

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Within the skeletal system, the structure of a bone, or osseous tissue, can be exemplified in a long bone, like the femur, where there are two types of osseous tissue: cortical and cancellous.
51.7K
Bone Remodeling01:40

Bone Remodeling

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Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
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Compact Bone01:27

Compact Bone

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Most bones contain compact and spongy osseous tissue, but their distribution and concentration vary based on the bone's overall function.
Compact bone, also called cortical bone, is the denser, stronger of the two types of bone tissue. It is found under the periosteum and in the diaphyses of long bones, where it provides support and protection. The microscopic structural unit of compact bone is called an osteon, or haversian system. Each osteon is composed of concentric rings of calcified...
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Bone Disorders01:29

Bone Disorders

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Aging and its effect on bone remodeling is the most common cause of bone disorders. In young and healthy people, bone deposition and resorption happen at an equal rate to maintain optimal bone health.
Bone deposition is also affected by the levels of sex hormones like estrogen and testosterone that promote osteoblast activity and bone matrix synthesis. When the level of these hormones decreases due to aging, it causes a reduction in bone deposition. As a result, bone resorption by osteoclasts...
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Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
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Raman Microscopy and Bone.

Simon R Goodyear1, Richard M Aspden2

  • 1Arthritis and Musculoskeletal Medicine, Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK.

Methods in Molecular Biology (Clifton, N.J.)
|February 8, 2019
PubMed
Summary
This summary is machine-generated.

Raman microscopy offers a quick, non-damaging way to analyze bone's chemical makeup. This technique allows simultaneous measurement of mineral and collagen, enhancing bone studies.

Keywords:
Bone chemistryMechanical properties.RamanSpectroscopy

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Area of Science:

  • Biomaterials Science
  • Chemical Analysis
  • Skeletal Biology

Background:

  • Bone tissue is a complex composite material crucial for skeletal function.
  • Understanding the chemical composition of bone is vital for diagnosing and treating skeletal diseases.
  • Current analytical techniques may be destructive or require extensive sample preparation.

Purpose of the Study:

  • To evaluate Raman microscopy as a method for characterizing bone tissue.
  • To demonstrate the capability of Raman microscopy for simultaneous analysis of bone's mineral and collagen components.
  • To highlight the advantages of Raman microscopy for bone research.

Main Methods:

  • Utilized Raman microscopy, a non-destructive optical technique.
  • Employed modern Raman instruments with integrated software for data acquisition.
  • Focused on minimal sample preparation requirements.

Main Results:

  • Raman microscopy successfully measured chemical properties of both mineral and collagen in bone simultaneously.
  • The technique demonstrated a spatial resolution of approximately one micron.
  • Acquired standard information required for most bone studies.

Conclusions:

  • Raman microscopy is a valuable, non-destructive tool for bone characterization.
  • Its ability to analyze mineral and collagen concurrently simplifies bone studies.
  • The technique requires minimal sample preparation, making it an efficient addition to the bone analysis toolbox.