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Updated: Jan 29, 2026

RNAi Mediated Gene Knockdown and Transgenesis by Microinjection in the Necromenic Nematode Pristionchus pacificus
Published on: October 16, 2011
Preparation of malto-oligosaccharides with specific degree of polymerization by a novel cyclodextrinase from
Hangyan Ji1, Yuxiang Bai1, XiaoXiao Li1
1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China.
Abstract:
An open reading frame from Palaeococcus pacificus was discovered by genomic analysis. The recombinant PpCD was proved as novel extremely thermal-stable cyclodextrinase with potential on production of malto-oligosaccharides with specific degree of polymerization. This enzyme has extremely high substrate specificity toward cyclodextrins, while the rates of hydrolysis toward macromolecular or linear substrates such as starch, pullulan and amylose were pretty slow. The optimal pH and temperature of PpCD were determined as 6.0 and 95 °C respectively. It was confirmed that PpCD could produce malto-oligosaccharides with a certain degree of polymerization in consistent with that of cyclodextrin substrates. In this study, the preparation of high purity maltoheptaose was achieved and the suitable β-cyclodextrin concentration was about 8-10%. Therefore, PpCD may have a potential application in the production of specific degree of polymerization malto-oligosaccharides.
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