[Reference values of platelet aggregometry in healthy subjects.]

Area of Science:

• Clinical Biochemistry
• Hematology
• Diagnostic Laboratory Science

Background:

• Platelet aggregation is a critical hemostatic process.
• Establishing reliable reference intervals for platelet function tests is crucial for accurate clinical diagnosis.
• Variability in platelet aggregation measurements necessitates standardized reference values.

Purpose of the Study:

• To determine the average values and reference intervals for platelet aggregation in healthy individuals.
• To provide normative data for platelet aggregation using adenosine-5`-diphosphate (ADP) as an agonist.
• To establish reference standards for the AggRAM optical aggregometer in a clinical diagnostic setting.

Main Methods:

• Utilized the AggRAM optical aggregometer for platelet aggregation assays.
• Measured platelet aggregation in healthy individuals stimulated with two concentrations of adenosine-5`-diphosphate (5 µg/ml and 10 µg/ml).
• Calculated reference intervals for both platelet aggregation percentage and the area under the aggregation curve.

Main Results:

• Reference intervals for platelet aggregation with 5 µg/ml ADP were 67.4-92.5% and 54.5-85.3% (area under the curve).
• Reference intervals for platelet aggregation with 10 µg/ml ADP were 76.8-97.2% and 68.8-90.4% (area under the curve).
• These results provide specific quantitative data for platelet function.

Conclusions:

• The established reference intervals are suitable for use in clinical diagnostic laboratories.
• The findings support the use of the AggRAM optical aggregometer for routine platelet function testing.
• This study contributes to standardized diagnostic practices in hematology.